Microinjection - 9781588298843

Cellular microinjection techniques have matured such that they can now be employed for a wide variety of biological laboratory and clinical purposes, including the injection of antibodies, fusion proteins, DNA, and in vitro-synthesized RNA or even the production of transgenic animals. Carroll (Florida Institute of Technology) presents 15 chapters describing modern and classic techniques of microinjection. Examples of specific topics addressed include expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation, combining microinjection and immunoblotting to analyze MAP kinase phosphorylation in single starfish oocytes and eggs, analysis of signaling pathways in zebrafish development by microinjection, protein inhibition by microinjection and RNA-mediate interference in tissue culture cells, DNA delivery by microinjection for the generation of recombinant mammalian cell lines, germline transformation of Caenorhabditis elegans by injection, quantitative microinjection of mouse oocytes and eggs, microinjection of follicle-enclosed mouse oocytes, function study of regulatory genes in the sea urchin embryo, exploring the cytoskeleton during intracytoplasmic sperm injection in humans, and somatic cell nuclear transfer in the mouse. Each chapter includes lists of necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and pitfalls. Annotation ©2009 Book News, Inc., Portland, OR (booknews.com)