Culture of Animal Cells - 9781118873656

Culture of Animal Cells: A manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this text is the updated version of this text addressing recent developments in the field as well as the basic skills and protocols.

This edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides and introduction to new subtopics and mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to limit the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition, provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of the animal cells. This text is a resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees, and technicians.

Includes clearly understood protocols, lists the requisite materials for all basic procedures, and provides guidelines on safety and bioethics

Specialized procedures explained in similar detail to allow use without further supplementation from the primary literature

Includes additional series of minireviews on topics such as stem cells and regenerative medicine, 3D culture, high throughput screening and authentication essays

Supplementary material with descriptive diagrams and photographs

Contains instructions on preparing and equipping the cell culture laboratory, including preparation and sterilization of media, and reagents, with lists of materials and equipment sources

Contains a training chapter with detailed training exercises, some with experimental components

Provides cautionary advise on instability, contamination, and misidentification with appropriate protocols to enable correct quality control