Inflammation and Cancer - 9781603275293

Growing evidence suggests a strong link between inflammatory processes and oncogenesis, according to Kozlov (National Cancer Institute), which has naturally led to increased medical interest in the infection-inflammation-cancer axis on a molecular level, as well as the search for novel markers that, once targeted, can selectively restrain the oncogenic drift triggered by inflammation. This volume contains 16 papers describing methodologies for examining both these areas. Topics include molecular analysis of genetic instability caused by chronic inflammation, inflammation-related aberrant patterns of DNA methylation, cDNA microarray analysis of inflammatory breast cancer signatures, induced nitric oxide synthase as a major player in the oncogenic transformation of inflame tissue, development of a cell-based assay to quantify the inflammatory potential of test substances and screen compound libraries for anti-cancer drug candidates in high-throughput format, quantitative measurement of cytokine expression in synoviocytes derived from rheumatoid arthritis patients, investigating macrophage and malignant cell interactions in vitro, and the molecular basis of histone deacetylase inhibitors as new drugs for the treatment of inflammatory diseases and cancer. Each contribution includes an introduction, lists of necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and known pitfalls. Annotation ©2009 Book News, Inc., Portland, OR (booknews.com)