Pcr - 9780415355476

McPherson (biology, U. of Leeds) and Möller (mathematics and natural sciences, U. of Stavenger) provide an introductory text for undergraduates, graduates and researchers who wish to use the polymerase chain reaction (PCR), a DNA-copying technique, in their experiments. Focusing on fundamental principles and lab procedures, they introduce the concepts behind PCR and how it works, then cover the practicalities of reagents and instrumentation, optimization of PCR, analysis, sequencing, in vitro expression of PCR products, purification and cloning of PCR products, analysis of gene expression, cloning genes by PCR, and genome analysis. This edition also includes a new chapter on real-time PCR and more information on complex mutagenesis and applications in genomics and proteomics. Annotation ©2006 Book News, Inc., Portland, OR (booknews.com)