Real-time PCR - 9780415377348

Real-time polymerase chain reaction shifts emphasis from the end product of the biomedical technique to the whole course of the PCR process and has established itself, according to editor Dorak (School of Clinical Medical Sciences-Child Health, Newcastle U., UK), as the "most sensitive and specific quantitative PCR method." He presents a laboratory "how-to" guide to real-time PCR, first presenting chapters on the basics of data analysis, quantification, normalization, and principles of primer and probe design. This first section of the book concludes with chapters on applied aspects such as high-resolution melting analysis. The second half of the text covers applications in more detail and offers chapters on quantitative analyses of DNA methylation, mitochondrial DNA analysis, real-time immuno-PCR, clinical microbiology and virology, solid organ transplant modeling,real-time-PCR applications in hematology, and prenatal diagnosis of monogenic disease caused by single nucleotide changes. Annotation ©2006 Book News, Inc., Portland, OR (booknews.com)