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Clostridium Difficile - 9781603273640

Un libro in lingua di Mullany Peter (EDT) Roberts Adam P. (EDT) edito da Springer Verlag, 2010

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While there has been an emergence of highly transmissible and antibiotic-resistant Clostridium difficile, which causes diarrhea and more serious intestinal conditions, there has also been a dramatic increase in available molecular tools to study this major nosocomial pathogen. Mullany and Roberts (UCL Eastman Dental Institute, London) compile 15 chapters on the causes of C. difficile disease, and isolation and culture methods and animal models for studying its typing, biochemistry, genomics, and immunology. International contributors present detailed descriptions of how to carry out, e.g., polymerase chain reaction (PCR)-ribotyping, transcription of the toxin genes, and gene knockout to generate mutations. Protocols include a general overview, lists of materials, methods, notes, references, and workflow/other illustrations. Annotation ©2010 Book News, Inc., Portland, OR (booknews.com)

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